大黄素和大黄酸的体外遗传毒性评价

doi:10.3969/j.issn.1004-616x.2011.01.017

›› 2011, Vol. 23 ›› Issue (1): 65-067.doi: 10.3969/j.issn.1004-616x.2011.01.017

• 检测研究 • Previous Articles Next Articles

Evaluation of in Vitro genotoxicity of emodin and rhein

ZHU Qin-zhuCHEN Wei，ZHANG Li-shi*

Department of Nutrition and Food Hygiene, West China School of Public Health, Sichuan University, Chengdu 610041, Sichuan, China

Received:2010-09-28 Revised:2010-10-22 Online:2011-01-30 Published:2011-01-30
Contact: ZHANG Li-shi

Abstract

Abstract: To evaluate in vitro genotoxicity of emodin and rhein. METHODS: The WTK1 cells were treated by emodin and rhein of different concentrations (20, 40, 80, 120 μg/ml) and then the comet assay, in vitro micronucleus test and TK gene mutation assay were conducted simultaneously. The solvent control and positive control (mthylmethane sulfonate，MMS) groups were included in the study. RESULTS: Positive results were found at the concentrations of 120 μg/ml and 80 μg/ml of emodin in comet assay and tk gene mutation analysis. High dose of rhein (120 μg/ml) also showed weak mutagenicity in TK gene mutation analysis. CONCLUSION: Under the present experimental conditions, emodin and rhein showed weak mutagenicity.

Key words: emodin, rhein, genotoxicity, in vitro micronucleus test, comet assay, TK gene mutation analysis.

ZHU Qin-zhuCHEN Wei，ZHANG Li-shi*. Evaluation of in Vitro genotoxicity of emodin and rhein[J]. , 2011, 23(1): 65-067.

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Evaluation of in Vitro genotoxicity of emodin and rhein

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